Anti-ExoS | Exoenzyme S
SKU: BTL-AGR-A-00050 | Host: Chicken | Brand: Agrisera
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| Cat Number | AS05 056 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 100 µl |
| Description | Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells. |
| Applications | Western blot (WB) |
| Immunogen | amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations. |
| Clonality | Polyclonal |
| Host | Chicken |
| Form | Liquid |
| Molecular Weight | 48 kDa |
| Dilution Range | 1 : 5000 (WB) |
| Uniprot Number | Q51449 , PA3841 |
| Purity | Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide. |
| Specie Reactivity | Pseudomonas aeruginosa |
| Expected Reactivity | Pseudomonas aeruginosa |
| Storage | Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |