Anti-EGFP | Enhanced Green Fluorescence Protein
SKU: BTL-AGR-A-02348 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS21 4583 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 100 µg |
| Description | EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. |
| Applications | Immunocytochemistry (ICC), Western blot (WB) |
| Immunogen | Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Molecular Weight | 27 kDa (for GFP without fusion partner) |
| Dilution Range | 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB) |
| Purity | Immunogen affinity purified in PBS pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide. |
| Specie Reactivity | GFP, EGFP |
| Reconstitution | For reconstitution add 100 µl of sterile water, ultrapure water |
| Storage | Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened |
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| Note | The product is for research use only |